_em_assembly.id 1 _em_assembly.entry_id 2BK2 _em_assembly.name 'pneumolysin liposome prepore complex' _em_assembly.aggregation_state 'single particle' _em_assembly.composition 'pneumolysin bound to liposomes' _em_assembly.num_components 2 _em_assembly.resolution_published 28 _em_entity_assembly.id 1 _em_entity_assembly.assembly_id 1 _em_entity_assembly.type protein _em_entity_assembly.name 'pneumolysin prepore' _em_entity_assembly.ebi_organism_scientific 'Streptococcus pneumoniae' _em_entity_assembly.ebi_engineered yes _em_entity_assembly.ebi_expression_system 'escherichia coli' _em_entity_assembly.id 2 _em_entity_assembly.assembly_id 1 _em_entity_assembly.type lipid _em_entity_assembly.name liposome _em_entity_assembly.details 'prepared by dialysis or extrusion of cholesterol, phosphatidylcholine, and dicetyl phosphate in a 10:10:1 molar ratio with a final lipid concentration of 2 mM (dialysis) or 4 mM (extrusion)' _em_entity_assembly_list.id pneumolysin _em_entity_assembly_list.entity_id 1 [<--this ties to the _entity.id] _em_entity_assembly_list.entity_assembly_id 1 _em_entity_assembly_list.number_of_copies 31 _em_entity_assembly_list.mol_wt 52,000 _em_entity_assembly_list.mol_wt_method 'calculated from translated gene sequence'