2004-06-03 2004-09-07 false Petr G. Leiman, Paul R. Chipman, Victor A. Kostyuchenko, Vadim V. Mesyanzhinov, Michael G. Rossmann Three-dimensional rearrangement of proteins in the tail of bacteriophage T4 on attachment to a host cell CELL 118 419 429 emd_1086.map float (32-bit) 256 256 256 -128 -128 -127 127 127 128 256 256 256 1006.810 1006.810 1006.810 90.000 90.000 90.000 X Y Z -11.22305 14.63493 0.00000 0.00000 1

Createdby MAPMAN V. 010122/7.0at Tue Mar 23 11:36:56 2004forA. Nonymous// Created by IMAGIC: CCP4 image = angles-aprq-unbin-1-deg-no-ctf-align-4A.map03-06-20 15:45:49 //

3.93285 3.93285 3.93285 CryoEM density of the T4 tail with contracted sheath and the baseplate in the star conformation. T4 phages treated with 3 M urea singleParticle 1 220 220 Estimate virus bacteriophage T4 phage T4 220 220

treated with 3 M urea

BACTERIA(EUBACTERIA) Escherichia coli VIRION STRAIN 02.043.0.01.005 false false ETHANE 100 FEI/PHILIPS CM300FEG/T Side entry liquid nitrogen-cooled cryo specimen holder GATAN LIQUID NITROGEN 2002-01-06 300 SPOT SCAN BRIGHT FIELD 500 3400 0 0 45000 47000 FIELD EMISSION GUN 20 FEI 100 Kodak SO163 film 100 3.93285 8 ZEISS SCAI

The components were separately fitted using Situs v.2.0

H2O

5 7.5 200 mesh cupper grid back-projection Spider Each particle 16 FSC at 0.5 cut-off theta 45 degrees, phi 180 degrees 1965